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KMID : 1094720000050030181
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 3 p.181 ~ p.185
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Optimal conditions for the production of exopolysaccharide by marine microorganism Hahella chejuensis
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Ko Sung-Hwan
Lee Hyun-Sang
Park Shin-Hye
Lee Hong-Kum
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Abstract
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A marine microorganism, strain 96CJ10356 produced exopolysaccharide, designated as EPS-R. To optimize culture conditions for the production of EPS-R, carbon and nitrogen sources, mineral salts, temperature, and pH were examined. From this study, STN medium for the production of EPS-R was suggested as follows; sucrose 20 g, tryptone 10 g, NaCl 10 g, MgSO4 5 g, CaCl2 1 g, KH2PO4 76 mg, K2HPO4 83 mg, FeCl2 5 mg, MnCl2 1 mg, NaMoO4 1 mg, and ZnCl2 1 mg per liter at pH 7.0. About 9.23 g/L of EPS-R was obtained from STN medium after cultivation for 120 h at 25°C in a 5-liter jar fermentor with an aeration rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased with the production of EPS-R and the maximal values were 415 cP and 1400 unit/mL against 0.5% activated carbon, respectively.
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KEYWORD
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exopolysaccharide, marine microorganism, culture conditions, Hahella chejuensis
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